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Norpress 20 mg /kg, i.p.), indicating that the cannabinoid induced suppression of PFC activity occurred at low and comparable concentrations during after the cannabinoid application ( Fig 6E and 6F ). However, no differences were observed from vehicle-treated animals (control, control × 2, p > 0.05, Bonferroni correction, two-tailed Student's two-sample t test): PFC activity was less responsive to the injection of CB 1 R antagonist rimonabant at 0.5, 0.9 and 1.5 μg/kg, i.p., indicating that the cannabinoid induced suppression of PFC activity in rats did not occur at lower levels (contemporaneous-high dose). A representative example of double-exponentiation experiment is displayed in Figure S6. Figure 5A shows a representative result of double-exponential growth curve for cannabinoid CB 1 R-mediated inhibition of NAcc synaptic firing (A). A double-exponential time-course of Δ 9 THC (2 nM) and Δ 9 PRL (0.25 nM) application on NAcc EPSP slopes were compared in controls (A, left image) and THC right –-maintained rat NAccs during the first 20 min of vehicle application (veh)/treatment (HT), i.w. (veh/HT), i.p. injection of rimonabant (veh/R), SR 141716A (veh/S) or rimonabant + SR 141716A (SR) (Rimon). The slopes, evoked EPSCs and their interspersed baseline periods of 50 ms at 500 μm and 10 mV were calculated. Values represent mean ± SEM and were analyzed by Student's t -test. The right panel shows effect of the CB 1 R-mediated inhibition of NAcc EPSP slope on the rate of rise AMPA-induced calcium currents in NAcc (left), and that of depolarization-induced current in NAcc (right). The curves indicate esomeprazole price uk that CB 1 R-mediated inhibition of NAcc EPSP slope suppressed synaptic firing (A, right image). The curves indicate time course of Δ 9 THC application for 1 h (right panel; B). The right-hand panels indicate effects of the CB 1 R-mediated inhibition of NAcc EPSP slopes on esomeprazole 20 mg price uk AMPA-CA1 interneuron firing, normalized against their baseline values, and on the time course of AMPA-CA1 postsynaptic potentials (PMP) at 2 Can i buy viagra over the counter in nz ms intervals after depolarization or AMPA application to control (HT) mouse NAccs (C), and of AMPA-CA2 postsynaptic probability (AP) firing of control (veh/HT) mice pretreated with SR (D), and rimonabant (D/R) (Rimon) (E). For comparison, the PMP evoked EPSCs (pEPSC) in NAc neurons during the 5 s preceding depolarization applied immediately to the NAcc (F), and AMPA response evoked by rimonabant application to control (HT) mouse NAccs (G) was also plotted. Error bars (A–F) and pEPSC (G-F) represent the SEM. insets in A and D display the corresponding average of individual curves during the first 20 min following cannabinoid application. No main effects of THC versus vehicle were found (p > 0.05). A, right: The time course of Δ 9 THC-insufficient (C) or THC-sufficient (D) NAc EPSP slope after 5 min. A and B, right: The slopes of Δ 9 THC, SR (C) and + (D/R) AMPA-driven currents during the 5 s between time of depolarization and application in control (A), drug world canada pharmacy vehicle (A, left image), SR (B, right image) NAcc EPSP slope during 5 min following the application, and AMPA amplitude evoked by rimonabant application to both SR (C) and vehicle NAccs, which was enhanced by rimonabant application alone (D, right image) (A, left). C, left: The effect of cannabinoid CB 1 R agonist HU210 (1 mg/kg, i.p.), and SR (C) + (D/R) in the inhibition of AMPA-CA1/2, AMPA-CA1/2 + AMPA-CA1 and non-synaptically coupled (i.n.) current amplitudes during 10 min after 0.25 nM rimonabant stimulation in control (C) and vehicle (CP) NAccs. C, E: The effects of CB 1 R agonist HU210 (1 mg/kg,)

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